Label-free quantitation: A new glycoproteomics approach
نویسندگان
چکیده
منابع مشابه
Label-free Quantitative Proteomics and N-Glycoproteomics Analysis of KRAS-activated Human Bronchial Epithelial Cells*□S
Mutational activation of KRAS promotes various malignancies, including lung adenocarcinoma. Knowledge of the molecular targets mediating the downstream effects of activated KRAS is limited. Here, we provide the KRAS target proteins and N-glycoproteins using human bronchial epithelial cells with and without the expression of activated KRAS (KRAS). Using an OFFGEL peptide fractionation and hydraz...
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With the prospect of resolving whole protein molecules into their myriad proteoforms on a proteomic scale, the question of their quantitative analysis in discovery mode comes to the fore. Here, we demonstrate a robust pipeline for the identification and stringent scoring of abundance changes of whole protein forms <30 kDa in a complex system. The input is ~100-400 μg of total protein for each b...
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Mass spectrometry (MS)-based shotgun proteomics allows protein identifications even in complex biological samples. Protein abundances can then be estimated from the counts of MS/MS spectra attributable to each protein, provided that one corrects for differential MS-detectability of the contributing peptides. We describe the use of a method, APEX, which calculates Absolute Protein EXpression lev...
متن کاملLabel-Free Absolute Quantitation of Oligosaccharides Using Multiple Reaction Monitoring
An absolute quantitation method for measuring free human milk oligosaccharides (HMOs) in milk samples was developed using multiple reaction monitoring (MRM). To obtain the best sensitivity, the instrument conditions were optimized to reduce the source and postsource fragmentation prior to the quadrupole transmission. Fragmentation spectra of HMOs using collision-induced dissociation were studie...
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Mass spectrometry is used routinely for large-scale protein identification from complex biological mixtures. Recently, relative quantitation approach on the basis of spectra count has been applied in several cancer proteomic studies. In this review, we examine the mechanism of this technique and highlight several important parameters associated with its application.
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2009
ISSN: 1044-0305,1879-1123
DOI: 10.1016/j.jasms.2009.01.013